System using gene editing technology

CRISPR Cas system

  • Accurately recognize and bind the target DNA
  • Cas protein and guide RNA complex
  • CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) : Viral immune system first discovered in microorganisms
  • Minimize off-target cleavage frequency

Protein engineering

GeneCker CRISPR Cas9 system​

Development of a new Cas9 that can distinguish On-Target and Off-Target at the 1 base pair (bp) level by modifying the amino acid residues for each domain of the existing Cas9 = gcCas9


The gcCas9 developed by GeneCker accurately detects the differences in a single base.

-> No off-target was found in the whole genome

  • Off-target was not observed on the whole genome
  • Most precise CRISPR system → highly utilization as a genetic therapy


Necessity and difficulty of mutant detection in liquid-biopsy

Existing diagnostic kits have limitations in detecting trace amounts of mutant DNA contained in liquid-biopsy

GeneCker CRISPR/Cas9 system removes normal genes from blood and amplifies target mutant genes only. And it emphasizes mutant gene.

Sager Sequencing
Next Generation Sequencing (NGS)
Real-time PCR