CRISmono™ NRAS
CRISmono™ NRAS
PRODUCT SPECIFICATION
Input DNA
gDNA ≥ 20 ng
Platform
Sanger sequencing
* ABI Genetic Analyzer recommended
Target size
Size : 221 bp / Gene : NRAS
Applications
SNV (p.G12X, p.G13X)
PERFORMANCE EVALUATION
KEY FEATURES
Diagnostic kit based on Sanger sequencing
NRAS gene mutation detection kit based on Sanger sequencing
CRISPR/Cas9 system
Genetic scissors cut wild-type DNA and amplify mutant DNA
High sensitivity
Genetic scissors enhance the detection limits of Sanger sequencing
Convenience of diagnosis
Available for periodic monitoring and cusomization
PROCESS
- 01 gDNA prep
- 02 In vitro Cleavage
- 03 PCR Amplification
- 04 Clean up
- 05 Sanger Sequencing
- 06 Data Analysis
Whole blood
gDNA prep
In vitro Cleavage
PCR Amplification
Clean up
Sanger Sequencing
Data Analysis